- Smeared DNA Agarose Gel Electrophoresis Troubleshooting {mosloadposition advert1} If your DNA has smeared on your agarose gel electrophoresis you may have the following problems: You loaded and ran degraded DNA. Make sure you run Avoid nuclease contamination. You loaded too much DNA into the gel well. Decrease the amount of DNA you are loading into each lane. Don't exceed [...]
- Troubleshooting Faint or No DNA Bands on Gel How to Troubleshoot Faint or No DNA Bands on Agarose Gel Electrophoresis {mosloadposition advert1} If you have faint or no bands on the gel you may have the following gel running or visualization problems: You loaded insufficient quantity or concentration of DNA on the agarose gel. The solution is to increase [...]
- Gel Electrophoresis has the gel protocols and methods you need to separate out your favorite molecules whether DNA, RNA or Protein.
- DNA Gel Electrophoresis. Information on how to separate DNA deoxyribonucleic acid on an agarose or polyacrilamide gel. Separation of DNA by Gel Electrophoresis DNA is usually separated by agarose gel electrophoresis although polyacrilamide gel electrophoresis for DNA is also often done. Large DNA or RNA is usually done by agarose gel electrophoresis whereas smaller fragments of DNA [...]
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Electrokinetic Phenomena
Electrokinetic Phenomena
Electrokinetic Phenomena emphasizes the impact of methods such as capillary zone electrophoresis, capillary electrochromatography, and capillary gel electrophoresis on the analysis of biomolecules. This reference reveals the electrokinetic phenomena that underlie high-performance electro-based analytical tools and vividly depicts how electrodriven analytical tools revolutionize and expedite chemical, pharmaceutical, and biotechnological analysis. An authoritative overview, the book provides effective pathways for large-scale biomedical applications and describes how microfabricated and automated devices enhance and accelerate the analysis of biologically important molecules.
List Price: $ 226.95
Price: $ 56.48
The Proteome Revisited, Volume 63: Theory and practice of all relevant electrophoretic steps (Journal of Chromatography Library)
The Proteome Revisited, Volume 63: Theory and practice of all relevant electrophoretic steps (Journal of Chromatography Library)
The book deals with the theory and practice of all electrophoretic steps leading to proteome analysis, i.e. isoelectric focusing (including immobilized pH gradients), sodium dodecyl sulphate electrophoresis (SADS-PAGE) and finally two-dimensional maps. It is a reasoned collection of all modern, relevant, up-to-date methodologies leading to successful fractionation, analysis and characterization of every polypeptide spot in 2-D map analysis. It includes chapters on the most sophisticated mass spectrometry developments and it helps the reader in navigating through the most important databases in proteome analysis, including step by step tours in selected sites. Yet, this book’s unique strength and feature is the fact that it combines not only practice (in common with any other book on this topic) but also theory, by giving a detailed treatment on the most advanced theoretical treatments of steady-state techniques, such as isoelectric focusing and immobilized pH gradients. A lot of this theory is newly developed and presented to the public for the first time. Thus, this book should satisfy not only the needs of every day practitioners, but also the desires of the most advanced theoreticians in the field, who will surely appreciate the novel theories presented here.
Also the methodological section contains several as yet unpublished protocols, correcting some of the existing ones and showing the pitfall and limitations of even well ingrained protocols in proteome analysis, which are here critically re-evaluated for the first time.
List Price: $ 250.00
Price: $ 246.40
Proteome Research: Mass Spectrometry (Principles and Practice)
Proteome Research: Mass Spectrometry (Principles and Practice)
Recent advances in large scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Mass spectrometry is the method of choice for the rapid large-scale identification of these proteomes and their modifications. This is the first book to extensively cover the applications of mass spectrometry to proteome research.
List Price: $ 115.00
Price: $ 9.99
Gel Electrophoresis
Smeared DNA Agarose Gel Electrophoresis Troubleshooting
{mosloadposition advert1}
If your DNA has smeared on your agarose gel electrophoresis you may have the following problems:
You loaded and ran degraded DNA. Make sure you run Avoid nuclease contamination.
You loaded too much DNA into the gel well. Decrease the amount of DNA you are loading into each lane. Don't exceed [...]
Troubleshooting Faint or No DNA Bands on Gel
How to Troubleshoot Faint or No DNA Bands on Agarose Gel Electrophoresis
{mosloadposition advert1}
If you have faint or no bands on the gel you may have the following gel running or visualization problems:
You loaded insufficient quantity or concentration of DNA on the agarose gel. The solution is to increase [...]
Gel Electrophoresis has the gel protocols and methods you need to separate out your favorite molecules whether DNA, RNA or Protein.
Gel Electrophoresis Videos
El cumple 2 de May
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Novus Biologicals Visual Protocols: In phase 2 of the western blot procedure, you will learn how to load a gel and separate the proteins through electrophoresis, based upon protein weight. Additional help can be found in the support section of www.novusbio.com, through our live chat service, or by calling us directly to talk with our [...]
Video 2 de Moy