Demystifying SDS-Page

An instructional video aimed at teaching the process of SDS-PAGE to college students with basic biochemical understandings. (www.sdspage.net)
Video Rating: 4 / 5

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• Western Blot Visual Protocol: Phase 2: Protein Electrophoresis (SDS-PAGE)
  ...
• SDS-PAGE Preparation
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• How to Avoid a Leaky SDS-PAGE gel
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25 Comments

1.   mrpapparappa   |  Sunday, 02 May 2010 at 9:06 am

   U guys ROCK!!! Helped me a lot n keep em comin, please !!!

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2.   proteinP53   |  Sunday, 02 May 2010 at 9:50 am

   muy bueno felicidades

   van 5 estrellas

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3.   michaelatm2603   |  Sunday, 02 May 2010 at 9:57 am

   I’m taking molecular biology class right now. I’m new to this,too. I think we can sort that protein based on its molecular weight and its pH, so we can further analyze what does that protein do based on protein library. For the buffer, I think it’s a part of SDS Page itself. You can see they add it at the beginning.

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4.   TOTSDesign   |  Sunday, 02 May 2010 at 10:33 am

   Great job guys! Funny way to explain the “misterious world” of dear SDS-Page…See ya!

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5.   johnnieguy09   |  Sunday, 02 May 2010 at 10:53 am

   Nice video, but I can’t believe you are A) Wearing shorts in a laboratory and B) eating cake in a laboratory. Didn’t you learn anything in safety training? lol

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6.   aesopfables4   |  Sunday, 02 May 2010 at 11:50 am

   woot woot cheers for slb :]

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7.   rsobhee   |  Sunday, 02 May 2010 at 12:09 pm

   Thnx for posting a dumb loud music in the background.. it made it easier to hear what you were talking..

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8.   ahnatanha   |  Sunday, 02 May 2010 at 1:09 pm

   do i love you guyz or what!!

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9.   Arammik   |  Sunday, 02 May 2010 at 2:00 pm

   The “one last concept” is actually the main concept of the laemmli system. Instead of running around in the forest, you guys could have explained that for about 5 mins in the video.

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10.   Polarcupcheck   |  Sunday, 02 May 2010 at 2:31 pm

    You guys seem to explain it as a kind of electron shielding.

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11.   Polarcupcheck   |  Sunday, 02 May 2010 at 2:32 pm

    I like, and dislike, the explanation of the stacking. I get it in the context of this video, but I’m still worried that isn’t the whole story, and may even be false. I never took physical chemistry, so maybe that is where the explanation is.

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12.   ianm12   |  Sunday, 02 May 2010 at 3:23 pm

    Nice work!

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13.   dwang008   |  Sunday, 02 May 2010 at 4:16 pm

    cool video, i learned alot. sorry for what may like “noob” questions but the chloride ions and glycine buffers, where do they come from? are they part of the protein solution or the gel? also, what is the significance in knowing the molecular weight of protein? How do researchers utilize that information?

    Im taking an intro biochem class right now and we havent covered this but im pretty interested.

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14.   azncount   |  Sunday, 02 May 2010 at 4:48 pm

    Hey guys AWESOME video!!! Thanks so much for clearing up the confusion I had and yes that cake looks really yummy.

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15.   capkeez   |  Sunday, 02 May 2010 at 4:54 pm

    I want some of that cake. And awesome video. Cleared up some stuff for me. Thanks!

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16.   DYANNN8   |  Sunday, 02 May 2010 at 5:52 pm

    I don’t think it was confusing at all. It was very helpful. Thanks.

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17.   disinterested22   |  Sunday, 02 May 2010 at 6:36 pm

    he’s a giant!

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18.   justintime303   |  Sunday, 02 May 2010 at 7:21 pm

    judy is mad cute haha

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19.   eluminusunknown   |  Sunday, 02 May 2010 at 7:25 pm

    confusing video………….priceless

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20.   shadowfan117   |  Sunday, 02 May 2010 at 7:41 pm

    DONT READ THIS, MARK AS SPAM IF POSIBLE If you do not copy and paste this onto 10 videos your mom will die? in 4 hours

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21.   berlin45ers   |  Sunday, 02 May 2010 at 8:28 pm

    my prtein is kind a small hahahahhahaha

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22.   Fatmob79   |  Sunday, 02 May 2010 at 8:59 pm

    UC DAVIS…sweet. I used to live in Arlington Farms near the “gel” site.

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23.   eliash1988   |  Sunday, 02 May 2010 at 9:22 pm

    best explanatory video, helped me in lab report…..keep it up

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24.   samipisces   |  Sunday, 02 May 2010 at 9:33 pm

    Really very helpful for beginners. Thanks for uploading such a wonderful video.

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25.   BusinessTime49   |  Sunday, 02 May 2010 at 10:22 pm

    Very helpful!!!!

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