Gel Electrophoresis

Gel electrophoresis

Last Updated on Monday, 3 May 2010 08:34 Written by Administrator Monday, 3 May 2010 08:34

Setting up and running of a DNA gel using electrophoresis.
Video Rating: 5 / 5

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25 Comments

HawaiiZach | Monday, 03 May 2010 at 8:37 am
@gkpeter One reason I perform gel electrophoresis to identify the size of the DNA band is to determine if the primers I used in PCR (Polymerase chain reaction – used to amplify small quantities of DNA into the thousands) actually cut the DNA to the length I needed. If you are only focusing on looking at a certain section of DNA primers will “cut” the section out you need, then they will be amplified to the thousands using PCR. You can then run the product out on a gel to determine if it cut

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canaan1967 | Monday, 03 May 2010 at 8:54 am
How are the wells numbered?

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gkpeter | Monday, 03 May 2010 at 9:00 am
@ZScarabello There are lots of reasons to know the sizes. Verification of constructs, genotyping, cloning, etc. But the simple answer is, yes, you can extract the DNA from the gel if needed. I have done it several times.

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ZScarabello | Monday, 03 May 2010 at 9:35 am
Hi gkpeter,

I was wondering if you or anyone else here could explain to me why there is a need to identify sizes of DNA. What is the point of this exercise? Can you extract the DNA after it has been separated?

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ZOMIBErevolution | Monday, 03 May 2010 at 9:52 am
this is cool

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foeasy16 | Monday, 03 May 2010 at 10:27 am
daaaaamn this is a community college??? we dont even have these high quality molecular replication equipment and im at a university :-/

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noorchaudery | Monday, 03 May 2010 at 10:53 am
thnx 2 upload, well plz explain the function of EtBr in electrophoresis, basically

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Peachpassion9 | Monday, 03 May 2010 at 11:02 am
@gkpeter Well, thanks for the help. I appreciate it.

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gkpeter | Monday, 03 May 2010 at 11:44 am
@Peachpassion9 I don’t have a video for that yet, but plan on it. Maybe next week. You compare your band to the band on the marker. If they ran about the same length, then they should be about the same size.

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Peachpassion9 | Monday, 03 May 2010 at 12:41 pm
I have the lambda HindIII genome, but I don’t know how I calculate the size of the DNA fragments I compare it with.

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Peachpassion9 | Monday, 03 May 2010 at 1:06 pm
Do you have a video that shows this?

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gkpeter | Monday, 03 May 2010 at 1:13 pm
@Peachpassion9 Compare it to a DNA ladder or molecular weight marker. You can guesstimate it or graph the known mass vs. length of migration from the well. You graph it on semi-log graph paper.

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Peachpassion9 | Monday, 03 May 2010 at 2:05 pm
how do you calculate the size of DNA fragments in the cut DNA samples?

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jesca215 | Monday, 03 May 2010 at 2:40 pm
perfect

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kayouna | Monday, 03 May 2010 at 3:29 pm
A molecular weight marker (commonly known as the ladder) contains DNA strands with known sizes (in base pairs) and so allows you to determine the size of your samples by comparing them to that reference.

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proteinP53 | Monday, 03 May 2010 at 4:06 pm
very good

5/5

gracias por subir este video van 5 estrellas

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valkyriemissiles | Monday, 03 May 2010 at 4:07 pm
question. what is molecular weight marker? what does it do?

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sukiwooty | Monday, 03 May 2010 at 4:51 pm
can you talk a little about the results? or maybe type a few lines or something please??
cheers~

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deefromott | Monday, 03 May 2010 at 5:20 pm
Thank you.

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andythrash | Monday, 03 May 2010 at 5:41 pm
Great work there, were gonna do one next wednesday at school hope it will work!

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0749061 | Monday, 03 May 2010 at 6:18 pm
Wonderful

Good work .. thanx

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gkpeter | Monday, 03 May 2010 at 6:20 pm
No problem, if you need anything else explained, feel free to ask.

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gkpeter | Monday, 03 May 2010 at 6:30 pm
No problem, if you need anything else explained, feel free to ask.

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amazaan | Monday, 03 May 2010 at 6:53 pm
it is handly for basic level of understanding about gel electrophoresis. thank you!!

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bulletforthetrooper | Monday, 03 May 2010 at 7:43 pm
very useful. thank you

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